Ensaio sobre agentes potencialmente antimicrobianos em cateter

Abstract

Introduction: The use of technology in health care has become increasingly necessary, especially when this is aimed at the prevention, diagnosis, treatment of diseases and their rehabilitation - in this study, we used nanotechnology to minimize the development of infections as much as possible by Candida albicans, Candida parapsilosis, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli, arising from catheters inserted in hospitalized patients in addition to reduce the expenses of these hospitalizations. The nanoparticles coated with antibacterial and antifungal agents are indexed as a coating on the materials internally and/or externally, releasing the compound "in loco", thus reducing the risk of biofilm formation. Objectives: The general objective of this study was to synthesize and use organic and inorganic nanomaterials in cateter coating, to control microorganisms. Material and Methods: Nanoparticles film composed of: ZrO2 (Zirconium Dioxide), Fe3O4 (Iron Oxide), Ag (Silver), TiO2 (Titanium Dioxide) e o ZnO (Zinc Oxide), applied to catheter samples of silicone and steel disk. The nanocomposites were synthesized by the Chemistry Department of the Federal University of São Carlos (UFSCAR) - nChemi Engenharia de Materiais LTDA, with a complement of microbiological analysis at the Microbiology Laboratory of the Faculty of Medicine of São José do Rio Preto, SP - FAMERP to carry out the tests experiments. The characteristics of the nanoparticle film and the compounds present in the prototypes were evaluated. These compounds were analyzed for their ability to prevented or not prevent bacterial and/or fungal growth when in contact with experimental specimens. Results: Initially, it was observed that the silicone indexing has not occurred as expected, since the high temperatures required in specific adsorption steps would damage the silicone structure. Thus, the indexing was performed on a steel disk that demonstrated the inhibition of Staphylococcus aureus when in contact with Ag and Klebsiella pneumoniae when in contact with Ag and ZnO. Candida albicans was inhibited using ZnO + Ag, TiO2 + Ag and Ag and Candida parapsilosis was inhibited by Ag and ZnO. Conclusions: The compounds Ag, TiO2 + Ag, ZnO + Ag were the ones that presented total inhibition of the chosen microorganisms. It was observed that the specimens composed of stainless steel, presented the desired inhibitory action for the study and that the process of fixing and drying of the compounds indexed on the specimens, is extremely relevant for the antimicrobial action.